In addition to the 45 tracks listed below, Rock Band will also feature 13 songs ranging from a variety of established bands to up-and-coming independent artists that can be unlocked as you play through the game.

* Indicates a cover song, all others songs are original master recordings

• 1960s
  • "Gimme Shelter" Rolling Stones
• 1970s
• * “Train Kept a Rollin'” as made famous by Aerosmith
• "Won't Get Fooled Again" The Who
• “Foreplay/Long Time” Boston
• * "Mississippi Queen" as made famous by Mountain
• “Next to You” The Police
• "Suffragette City" David Bowie
• * "Paranoid" as made famous by Black Sabbath
• "Don't Fear the Reaper" Blue Oyster Cult
• "Blitzkrieg Bop" The Ramones
• "Highway Star" Deep Purple
• "Detroit Rock City" Kiss
• “Flirtin' With Disaster”
• Molly Hatchet
• * “Green Grass & High Tides” as made famous by The Outlaws
• * “Ballroom Blitz” as made famous by Sweet
• 1980s
• * "Tom Sawyer" - as made famous by Rush
• "Wanted Dead or Alive" Bon Jovi
• "Should I Stay or Should I Go" The Clash
• "Epic" Faith No More
• “Orange Crush” R.E.M.
• "Wave of Mutilation" Pixies
• "Next to You" The Police
• * “Run to the Hills” as made famous by Iron Maiden
• 1990s
• "Learn to Fly" Foo Fighters
• "Enter Sandman" Metallica
• "In Bloom" Nirvana
• "Vasoline" Stone Temple Pilots
• "Say It Ain't So" Weezer
• "Cherub Rock" Smashing Pumpkins
• "Creep" Radiohead
• "Sabotage" Beastie Boys
• "Celebrity Skin" Hole
• "I Think I'm Paranoid" Garbage
• "Black Hole Sun" Soundgarden
• 2000s
• "Main Offender" The Hives
• "Go With the Flow" Queens of the Stone Age
• "Reptilia" The Strokes
• "Are You Gonna Be My Girl" Jet
• "Here It Goes Again" OK Go
• "The Hand That Feeds" Nine Inch Nails
• "Maps" Yeah Yeah Yeahs
• "Dani California" Red Hot Chili Peppers
• “Welcome Home” Coheed & Cambria
• “Dead on Arrival” Fallout Boy
• “When You Were Young” The Killers
• “Electric Version" New Pornographers